Journal: bioRxiv

Article Title: Effector T cells in poorly perfused tumor regions exhibit a distinct signature of augmented IFN response and reduced PD-1 expression

doi: 10.1101/2024.07.05.601540

Figure Lengend Snippet: (A) GSEA of RNA-seq data shows upregulated IFN alpha and gamma response hallmarks in 2-NBDG Lo CD4 and CD8 TILs. (B) IFN alpha and IFN gamma response hallmark genes with highest differential expression (fold change Log2 > 1) in both CD4 and CD8 2-NBDG Lo vs 2-NBDG Hi effector TILs in the RNA-seq data. (C) Expression of the indicated genes in effector CD4 and CD8 TILs sorted by their 2-NBDG uptake in vivo . (D) Expression of the indicated genes in effector CD4 and CD8 TILs sorted by their 2-NBDG uptake in vivo, in wild-type and IFNAR1-deficient mice (IFNAR1KO). Results are from 1 to 2 independent experiments. Dots are connected by lines to better visualize differences between 2-NBDG Lo vs Hi cells within each individual tumor. Statistical significance was assessed with a paired t test. * P < 0.05; ** P < 0.01; *** P < 0.001. P values below 0.1 are also indicated.

Article Snippet: After 5 days, T lymphocytes were harvested, adjusted at 10 6 cells/ml in fresh medium (15 mM or 0.3 mM glucose) and restimulated with anti-CD3 and anti-CD28 antibodies plus IL-2 (5 ng/ml) for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free), Biolegend, catalog: 581302).

Techniques: RNA Sequencing, Quantitative Proteomics, Expressing, In Vivo

Journal: Cell research

Article Title: Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses.

doi: 10.1038/s41422-024-01060-w

Figure Lengend Snippet: Fig. 1 GLO2 is downregulated after immune activation in immune cells. a RNA-seq data profiling of metabolic enzymes in mouse macrophages stimulated by Sendai virus (SeV) (12 h, MOI = 1) or LPS (6 h, 100 ng/mL). The untreated control group (Medium) is set to a value of 1. b, c Q-PCR (b) and Immunoblotting (c) analysis of indicated gene expression in BMDMs infected by VSV (MOI = 1) or LPS for indicated time points. Unless noted otherwise, all results in this and other figures were representative of at least three independent experiments. Q-PCR data are normalized to hypoxanthine-guanine phosphoribosyltransferase (HPRT) levels, and the medium group is set to a value of 1. Unless noted otherwise, the error bars in this and all other panels denote SD. d, e scRNA-seq analysis of GLO2 levels in different immune cells from human PBMCs infected by influenza A virus (GSE243629) (d) or stimulated by LPS (GSE226488) (e). f Q-PCR and immunoblot analysis of indicated gene expression in BMDMs stimulated as indicated. HKCA heat-killed preparation of Candida albicans, MitoPQ MitoParaquat. g Q-PCR and immunoblot analysis of indicated gene expression in CD3+ T cells separated from human PBMCs and then stimulated by anti- CD3/CD28 beads for indicated time points. h GLO2 levels in the expression profiling data of leukocytes from 124 patients with mild or severe COVID-19 (GSE221234). i GLO2 levels in leukocytes from patients with different sepsis endotypes in COVID-19. ADA Adaptive, IFN Interferon, IHD Innate-Host-Defense, INF Inflammatory, NPS Neutrophilic-Suppressive.

Article Snippet: For stimulation of macrophages with cytokines, recombined mouse IFNα (10149-IF, R&D systems), IL-6 (406-ML, R&D systems), IL-1β (HY-P7073, MedChemExpress), IFNγ (HY-P700184AF, MedChemExpress), and TNFα (410-MT, R&D systems) were added to the medium for indicated time points.

Techniques: Activation Assay, RNA Sequencing, Virus, Control, Western Blot, Gene Expression, Infection, Expressing

Journal: The American Journal of Pathology

Article Title: Vascular Endothelial Growth Factor Acts Primarily via Platelet-Derived Growth Factor Receptor α to Promote Proliferative Vitreoretinopathy

doi: 10.1016/j.ajpath.2014.07.026

Figure Lengend Snippet: Antibodies, Neutralizing Agents, and Growth Factors/Cytokines Used in This Study

Article Snippet: R&D Systems α-IFN-γ nAb Mouse IFN-γ Per manuf.

Techniques:

Journal: The American Journal of Pathology

Article Title: Vascular Endothelial Growth Factor Acts Primarily via Platelet-Derived Growth Factor Receptor α to Promote Proliferative Vitreoretinopathy

doi: 10.1016/j.ajpath.2014.07.026

Figure Lengend Snippet: PVR-Associated Growth Factors and Cytokines in Vitreous

Article Snippet: R&D Systems α-IFN-γ nAb Mouse IFN-γ Per manuf.

Techniques: